LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3416..3512) /label=attL2 /note="attL2 (c)" misc_signal complement(3527..3546) /note="T7 Promoter/priming site (c)" primer_bind 3554..3570 /note="M13 Reverse priming site" gene 3683..4492 /note="Kanamycin resistance gene" rep_origin 4613..5286 /note="pUC origin" vector join(3424..5289,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..3412 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:132531 IMAGE:8143874" /tissue_type="Liver, PCR rescued clones" /clone_lib="NIH_MGC_291" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: GAAAGCCC" gene 692..3412 /gene="KLB" /db_xref="GeneID:152831" /db_xref="HGNC:15527" misc_feature 692..3412 /gene="KLB" /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 692..3412 /dnas_title="klotho beta" /gene="KLB" /codon_start=1 /product="klotho beta" /protein_id="AAI04872.1" /db_xref="GI:85396950" /db_xref="GeneID:152831" /db_xref="HGNC:15527" /translation="MKPGCAAGSPGNEWIFFSTDEITTRYRNTMSNGGLQRSVILSAL ILLRAVTGFSGDGRAIWSKNPNFTPVNESQLFLYDTFPKNFFWGIGTGALQVEGSWKK DGKGPSIWDHFIHTHLKNVSSTNGSSDSYIFLEKDLSALDFIGVSFYQFSISWPRLFP DGIVTVANAKGLQYYSTLLDALVLRNIEPIVTLYHWDLPLALQEKYGGWKNDTIIDIF NDYATYCFQMFGDRVKYWITIHNPYLVAWHGYGTGMHAPGEKGNLAAVYTVGHNLIKA HSKVWHNYNTHFRPHQKGWLSITLGSHWIEPNRSENTMDIFKCQQSMVSVLGWFANPI HGDGDYPEGMRKKLFSVLPIFSEAEKHEMRGTADFFAFSFGPNNFKPLNTMAKMGQNV SLNLREALNWIKLEYNNPRILIAENGWFTDSRVKTEDTTAIYMMKNFLSQVLQAIRLD EIRVFGYTAWSLLDGFEWQDAYTIRRGLFYVDFNSKQKERKPKSSAHYYKQIIRENGF SLKESTPDVQGQFPCDFSWGVTESVLKPESVASSPQFSDPHLYVWNATGNRLLHRVEG VRLKTRPAQCTDFVNIKKQLEMLARMKVTHYRFALDWASVLPTGNLSAVNRQALRYYR CVVSEGLKLGISAMVTLYYPTHAHLGLPEPLLHADGWLNPSTAEAFQAYAGLCFQELG DLVKLWITINEPNRLSDIYNRSGNDTYGAAHNLLVAHALAWRLYDRQFRPSQRGAVSL SLHADWAEPANPYADSHWRAAERFLQFEIAWFAEPLFKTGDYPAAMREYIASKHRRGL SSSALPRLTEAERRLLKGTVDFCALNHFTTRFVMHEQLAGSRYDSDRDIQFLQDITRL SSPTRLAVIPWGVRKLLRWVRRNYGDMDIYITASGIDDQALEDDRLRKYYLGKYLQEV LKAYLIDKVRIKGYYAFKLAEEKSKPRFGFFTSDFKAKSSIQFYNKVISSRGFPFENS SSRCSQTQENTECTVCLFLVQKKPLIFLGCCFFSTLVLLLSIAIFQRQKRRKFWKAKN LQHIPLKKGKRVVS" misc_feature 692..3412 /note="KLB coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ctatgacact ttccctaaaa actttttctg 721 gggtattggg actggagcat tgcaagtgga agggagttgg aagaaggatg gaaaaggacc 781 ttctatatgg gatcatttca tccacacaca ccttaaaaat gtcagcagca cgaatggttc 841 cagtgacagt tatatttttc tggaaaaaga cttatcagcc ctggatttta taggagtttc 901 tttttatcaa ttttcaattt cctggccaag gcttttcccc gatggaatag taacagttgc 961 caacgcaaaa ggtctgcagt actacagtac tcttctggac gctctagtgc ttagaaacat 1021 tgaacctata gttactttat accactggga tttgcctttg gcactacaag aaaaatatgg 1081 ggggtggaaa aatgatacca taatagatat cttcaatgac tatgccacat actgtttcca 1141 gatgtttggg gaccgtgtca aatattggat tacaattcac aacccatatc tagtggcttg 1201 gcatgggtat gggacaggta tgcatgcccc tggagagaag ggaaatttag cagctgtcta 1261 cactgtggga cacaacttga tcaaggctca ctcgaaagtt tggcataact acaacacaca 1321 tttccgccca catcagaagg gttggttatc gatcacgttg ggatctcatt ggatcgagcc 1381 aaaccggtcg gaaaacacga tggatatatt caaatgtcaa caatccatgg tttctgtgct 1441 tggatggttt gccaacccta tccatgggga tggcgactat ccagagggga tgagaaagaa 1501 gttgttctcc gttctaccca ttttctctga agcagagaag catgagatga gaggcacagc 1561 tgatttcttt gccttttctt ttggacccaa caacttcaag cccctaaaca ccatggctaa 1621 aatgggacaa aatgtttcac ttaatttaag agaagcgctg aactggatta aactggaata 1681 caacaaccct cgaatcttga ttgctgagaa tggctggttc acagacagtc gtgtgaaaac 1741 agaagacacc acggccatct acatgatgaa gaatttcctc agccaggtgc ttcaagcaat 1801 aaggttagat gaaatacgag tgtttggtta tactgcctgg tctctcctgg atggctttga 1861 atggcaggat gcttacacca tccgccgagg attattttat gtggatttta acagtaaaca 1921 gaaagagcgg aaacctaagt cttcagcaca ctactacaaa cagatcatac gagaaaatgg 1981 tttttcttta aaagagtcca cgccagatgt gcagggccag tttccctgtg acttctcctg 2041 gggtgtcact gaatctgttc ttaagcccga gtctgtggct tcgtccccac agttcagcga 2101 tcctcatctg tacgtgtgga acgccactgg caacagactg ttgcaccgag tggaaggggt 2161 gaggctgaaa acacgacccg ctcaatgcac agattttgta aacatcaaaa aacaacttga 2221 gatgttggca agaatgaaag tcacccacta ccggtttgct ctggattggg cctcggtcct 2281 tcccactggc aacctgtccg cggtgaaccg acaggccctg aggtactaca ggtgcgtggt 2341 cagtgagggg ctgaagcttg gcatctccgc gatggtcacc ctgtattatc cgacccacgc 2401 ccacctaggc ctccccgagc ctctgttgca tgccgacggg tggctgaacc catcgacggc 2461 cgaggccttc caggcctacg ctgggctgtg cttccaggag ctgggggacc tggtgaagct 2521 ctggatcacc atcaacgagc ctaaccggct aagtgacatc tacaaccgct ctggcaacga 2581 cacctacggg gcggcgcaca acctgctggt ggcccacgcc ctggcctggc gcctctacga 2641 ccggcagttc aggccctcac agcgcggggc cgtgtcgctg tcgctgcacg cggactgggc 2701 ggaacccgcc aacccctatg ctgactcgca ctggagggcg gccgagcgct tcctgcagtt 2761 cgagatcgcc tggttcgccg agccgctctt caagaccggg gactaccccg cggccatgag 2821 ggaatacatt gcctccaagc accgacgggg gctttccagc tcggccctgc cgcgcctcac 2881 cgaggccgaa aggaggctgc tcaagggcac ggtcgacttc tgcgcgctca accacttcac 2941 cactaggttc gtgatgcacg agcagctggc cggcagccgc tacgactcgg acagggacat 3001 ccagtttctg caggacatca cccgcctgag ctcccccacg cgcctggctg tgattccctg 3061 gggggtgcgc aagctgctgc ggtgggtccg gaggaactac ggcgacatgg acatttacat 3121 caccgccagt ggcatcgacg accaggctct ggaggatgac cggctccgga agtactacct 3181 agggaagtac cttcaggagg tgctgaaagc atacctgatt gataaagtca gaatcaaagg 3241 ctattatgca ttcaaactgg ctgaagagaa atctaaaccc agatttggat tcttcacatc 3301 tgattttaaa gctaaatcct caatacaatt ttacaacaaa gtgatcagca gcaggggctt 3361 cccttttgag aacagtagtt ctagatgcag tcagacccaa gaaaatacag agtgaCCAGC 3421 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 3481 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 3541 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 3601 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 3661 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 3721 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 3781 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 3841 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 3901 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 3961 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 4021 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 4081 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 4141 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 4201 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 4261 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 4321 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 4381 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 4441 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 4501 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 4561 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 4621 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 4681 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 4741 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 4801 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 4861 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 4921 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 4981 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 5041 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 5101 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 5161 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 5221 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 5281 TCACATGTT //